Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (16): 186-189.doi: 10.11924/j.issn.1000-6850.2011-3733

Special Issue: 资源与环境

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Fingerprinting Patterns of SSR Markers Constructed for 33 Loquat [Eriobotrya japonica (Thunb.) Lindl.] Germplasms

  

  • Received:2011-12-09 Revised:2012-02-25 Online:2012-06-05 Published:2012-06-05

Abstract:

SSR sequences are the reproducible, stable, rich polymorphism DNA molecular markers. Fingerprint patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying polymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Malus and Pyrus. The results showed that, the uses of seven primers, Hi01d05F, Hi12f04F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07, could distinguish 33 loquat germplasm from one another, completely, providing an essential criterion for classification and identification of the loquat resources.