Abstract:

5’-UTR of AtNUDT10 was cloned by PCR from Arabidopsis thaliana and was inserted into pBAR-GUS to form a recombined construct pNUDT10P-GUS. The construct was then used to transform wide-type Arabidopsis thaliana by Agrobacterium tumefaciens mediating. A batch of transgenic Arabidopsis thaliana with 5’-UTR of AtNUDT10 was obtained through glufosinate screening. The results showed that, we had got the promoter of AtNUDT2 that was constitutive promoter. Furthermore, the expression of the promoter could not be induced by the Pst.DC3000 and Pst.DC3000 AvrB.