Abstract:

The aim was to select the best method of new male sterile line breeding, and then simplify and improve the breeding process. Two different methods, successive inbreeding to retain segregating strains of sterile plants and segregating strains of sterile plants by sib-mating, was used to obtain a new genetic male sterile lines. The homozygous effects of ornamental characteristics were compared among the two methods. Such characteristics as flower diameter, scape and height were investigated in different generations of Petunia hybrida Vilm. The average variation coefficients of BC2F2 generations produced by successive inbreeding and sib-mating were 8.26% and 6.13%, and that of BC3F2 generations were 4.20% and 2.15%, respectively. The variation coefficient of successive inbreeding dwindled and the purification of homozygous genotype was sped up by the increase of breeding generations. The variation coefficient of successive inbreeding was smaller than that of sib-mating, which suggested that successive inbreeding was the best method of breeding Petunia hybrida Vilm genetic male sterile line. Therefore, precedence mode had been found to speed up F1 generation selecting process in Petunia hybrida Vilm as its breeding technical basis.