Abstract:

In order to improve the efficiency and reduce the cost for culturing Arbuscular mycorrhizal fungi, a pot experiment 3×2 factor was conducted with Zea mays and Trifolium pretense L. as host plants and three AMF species, Glomus intraradices (G.i), Glomus mosseae (G.m) and Glomus versiforme (G.v) were inoculated under three different substrates (P1, P2 and P3) individually which composed by sand, grass carbon, perlite, vermiculite and peat. And designed in two nutrient supply levels (H0: no additional nutrition, H1: 10%Hoagland’s). Three indexs, AMF colonization, photosynthetic characteristics of the host plants and spore density were measured and thereby to select a best light substrate and and its corresponding concentration of different AMF. The results showed that P2 (vermiculite, grass carbon, sand=4:1:1) was best for G.m and G.i but P3 (perlite, vermiculite, grass carbon, sand=2:2:1:1) for G.v. It was not only because of better colonization, higher photosynthetic efficiency but also much more spores than other substrates. However, it was necessary to point out that the adding of nutrient solution dramatically reduced spore density of G.m, G.i and G.v by 43.51%-96.26%, 57.50%-99.39% and 27.79%-92.56%, respectively. What’s more, the colonization of three AMF species was also decreased to different extents. The mechanism needs to be further studied.