Abstract:

The objective of this study was to obtain the full length cDNA of dehydrin gene, and to conclude its role during endo-dormancy release in tree peony (Paeonia suffruticosa). The flower buds endured different time of chilling were used as materials, the full length cDNA of dehydrin gene was obtained using the method of Rapid Amplification of cDNA Ends (RACE) PCR, and its expression pattern were analyzed using real-time PCR. Full length cDNA of dehydrin gene was 1187 bp including a 831 bp open reading frame (ORF), 114 bp 5’ terminal untranslated region (UTR) and 242 bp 3’ terminal UTR followed by 26 bp polyA tail. The deduced amino acid sequence had two conservative K segments, which was the character of dehydrin gene family. According to the classification method of Close, it should be YSK2 type. Phylogenetic analysis indicated that the deduced psDHN-YSK2 was firstly clustered with that of grape. The expression of psDHN-YSK2 was steady up-regulated early in chilling requirement fulfillment. The full length cDNA of dehydrin gene was obtain and it expression trend was analyzed. All results suggested that it played an important role in the regulation of dormancy release in tree peony.