Abstract:

The aim was to reveal the potential value of the endophytes in Fritillaria przewalskii Maxim., diversity and bioactivities of alkaloid-metabolites derived from endophytic fungus were studied. Alkaloids from the fermentation products of endophytic fungus were detected by Dragendorff’s and thin layer chromatography (TLC). The agar-well diffusion method was applied to test the antibiotic activities. The screened strain was classified by morphological features combining with sequencing of internal transcribed spacer sequences (ITS). There were thirteen endophytes isolated from the healthy bulbs of F. przewalskii, of them the strain G6 had the ability to produce several alkaloid components. However, according to the TLC analysis, these alkaloids from strain G6 were not identical with standard substances or those from bulbus Fritillariae Cirrhosae. The crude extract of alkaloids played a positive role in inhibiting the growth of Staphyloccocus aureus and Enterococcus faecalis, significantly and moderately, respectively. With the help of morphological features and the information contained by ITS sequences, the strain G6 was identified as Fusarium sambbucinum. On the basis of above factors, it was most likely that the strain G6 had wide prospect as a kind of donor source in exploiting new antibiotic substances and novel alkaloids, even though it could not produce the associated-plant alkaloids.