Abstract:

The aim was to provide the theoretical basis for large scale propagation of adventitious root of Hypericum perforatum L. In this study, the adventitious root of Hypericum perforatum was used as the experimental material for suspension culture, to optimize the screening of medium types, sugar types and IBA concentration of adventitious root growth. Then the adventitious root of Hypericum perforatum growth was observed, fresh weight and dry weight were measured, dry matter and multiplication coefficient were calculated to determine the optimal suspension culture conditions. Different medium types had a greater impact on adventitious root of Hypericum perforatum growth, MS medium was the best factor to promote the growth of adventitious root; Sucrose significantly increased the fresh weight and dry weight of adventitious root of Hypericum perforatum, and the values reached 8.29 g, 0.46 g respectively. The fresh weight, dry weight, dry matter and multiplication coefficient of adventitious root of Hypericum perforatum had reached the maximum when the IBA concentration was 1.0 mg/L, the maximun values was 8.98 g, 0.49 g, 5.46% and 13.97 respectively. Adventitious root of Hypericum perforatum propagated best when the medium was MS+IBA 1.0 mg/L, sucrose as carbon source in suspension culture.