Abstract:

In order to identify the dehydrin gene of Populus hopeiensis and analyze the expression regularity under abotic stresses for the genetic regulation functions elucidation in stress, the primers were designed on the basis of previous work in our lab and the gene was amplified by PCR using cDNA library, the expression of the gene under abotic stresses was analyzed by semi-quantitative RT-PCR. The results indicated that dehydrin gene of P. hopeiensis was 856 nucleotides long and encoded a protein of 227 amino acid sequences. Sequencing and the homology analysis indicated that P. hopeiensis dehydrin gene was highly similar with dehydrin gene in Populus species. Consequently P. hopeiensis dehydrin gene could be named Phdhn1. Semi-quantitative RT-PCR expression analysis of Phdhn1 under drought, cold, heat, high salinity and ABA at different stress stage (0, 0.5, 1, 2, 5, 10, 24 h) showed that it was differentially expressed in response to drought, ABA and cold stresses. The study showed that Phdhn1 played an important role in abotic stresses of P. hopeiensis.