Abstract:

In order to study the effects of Bufonis parenteral on immunity effect of Newcastale disease vaccine, 240 one-day-old healthy chickens were randomly divided into control group and treatment group (low dosage groups, medium dosage groups and high dosage groups), each group had 60 chickens. The different concentration combining usage of Bufonis parenteral solutions and Newcastale disease vaccine were selected as the treatment group. At the 21st, 28th, 35th, 42nd, 49th and 56th day, using β-micromethod, tested ND-HI antibody in the serum; using MTT method, tested peripheral blood T-cell absorbance; using the method of weighing body weight and immune organ weight, tested immune organs index. The results showed that the ND-HI antibody level, peripheral blood T-cell transformation, thymus index, bursal index and spleen index of Bufonis parenteral groups were improved to varying degrees comparing to control group. The results indicated that Bufonis parenteral could improved the body humoral immune and cellular immune level, thus enhance Newcastle disease vaccine immune effect.