Abstract:

In hybrid rice breeding, new restore lines breed process should be promoted because of the restriction of the narrow genetic relationship. Yunnan Province, processing unique climate conditions, provided abundant of rice landraces which is the ideal resources for new cytoplasm type restore lines selection. In our research, 61 Yunnan landraces were used for test-crossing, and 6 lines were selected through the fertility restorer ability. Moreover, 5 specific primers which located inside restore gene locus were used for analyzing the genetic diversity of restorer gene locus among them and another 7 restorer lines. The results showed that the amplified bands types were difference among the 6 landraces. When compared to the 7 restorer lines, there was the same amplified band type (MW: 2.8 kb), which means the restorer gene existed in Yunnan landraces had a distant relationship with the restorer lines’ using in rice production. So it could be an efficient measure to exploit new restorer genes and resources from Yunnan rice landraces.