Abstract:

Nested PCR and fluorescent SYBR Green Ⅰ real time PCR were developed for detection of several foreign genes (CaMV35S, CP4EPSPS) in genetically modified soybean. The results showed that, nested PCR could detect CaMV35S in 1 ng/μL DNA and the detection limit was 100 ng/μL in the first PCR. SYBR Green Ⅰ real time PCR could detect CaMV35S, CP4EPSPS genes and the detection limit was 0.1 ng/μL for CaMV35S. Different kinds of soybean foodstuffs were detected by nested PCR and real time PCR, the results showed that, no CaMV35S gene was detected in all the foodstuffs. Therefore, nested PCR and the SYBR Green Ⅰ real time PCR were the effective methods for detecting GM soybean foodstuffs.