Abstract: In order to research the wheat apical merited and tillering node of Agrobacterium tumefactions -mediated transformation for the receptors of feasibility, genetic transformation of OsDREB gene from rice (Oryza sativa) into two wheat varieties mediated by Agrobacterium tumefactions was studied with wheat apical merited and tillering node as explants. The results showed that, both methods could obtain the PCR-positive transgenic plants: survival rate for T0 generation with apical merited was lower (30.02%) than that (100%) of T0 generation with tillering node injection, but transformation ratio (13.79) for the apical merited method was higher than that (3.25% ) of tillering node method. The optimum concentration for kanamycin treatment was 90 mg/L by the wild-type kanamycin-resistance screening. The optimum carbenicillin was 300 mg/L to inhibit the growth of Agrobacterium tumefactions during the co-culture by bud growing point infection method, and it not only ensured low contaminated rate, but also improved bud regeneration rate.