Abstract:

To establish a RNA interference technique in Scapharca Kagoshimensis for usage in research of gene function, double strand RNA was synthesized using in vitro transcription. The genes examined here using RT-PCR technique for determining the validity of RNA interference could be effectively knocked down at 48 h post injection of double strand RNA. The shortest effective time of RNA interference was found to be 48 h post injection when the gene expression was analyzed at different time points, i.e. 12, 48, 72, 96, 120 h after injection of dsRNA for examining the temporal effect of RNA interference, and the effective time could last up to 120 h. Moreover, the decrease of gene expression could be observed in different tissues, indicating that RNA interference could be induced systemically and the interference effect could be transported among different tissues. Here, we reported a stable RNA interference technology which could provided an effective molecular biological approach for the identification of gene function and antiviral research.