Abstract:

In order to understand the function of a gene, constructing the gene deletion mutant was the most common method. Analysis of the gene function through the determination of mutant characters to obtain a phenotype mutant, which determined by genotype was very important in biology research. 3 methods to construct gene mutant, such as transposon inserting mutant, homologous recombination and site-directed mutagenesis.The method of transposon insertion was used to construct the mutant library, homologous recombination was used to study the gene function, and site-directed mutagenesis was used to study the protein function. 3 methods emphasize a different aspect respectively. The combination of homologous recombination and site-directed mutagenesis would to be a new direction for gene mutations. Finally, the defects and shortcomings of gene mutation technology were discussed in this paper. The technology was proposed to be improved in the future.