Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (18): 179-184.doi: 10.11924/j.issn.1000-6850.2012-0888
Special Issue: 生物技术
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Abstract:
In order to understand the function of a gene, constructing the gene deletion mutant was the most common method. Analysis of the gene function through the determination of mutant characters to obtain a phenotype mutant, which determined by genotype was very important in biology research. 3 methods to construct gene mutant, such as transposon inserting mutant, homologous recombination and site-directed mutagenesis.The method of transposon insertion was used to construct the mutant library, homologous recombination was used to study the gene function, and site-directed mutagenesis was used to study the protein function. 3 methods emphasize a different aspect respectively. The combination of homologous recombination and site-directed mutagenesis would to be a new direction for gene mutations. Finally, the defects and shortcomings of gene mutation technology were discussed in this paper. The technology was proposed to be improved in the future.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2012-0888
https://www.casb.org.cn/EN/Y2012/V28/I18/179