Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (18): 179-184.doi: 10.11924/j.issn.1000-6850.2012-0888

Special Issue: 生物技术

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Research Advancement on 3 methods of Gene Mutation and Application in Microbes

  

  • Received:2012-03-14 Revised:2012-03-29 Online:2012-06-25 Published:2012-06-25

Abstract:

In order to understand the function of a gene, constructing the gene deletion mutant was the most common method. Analysis of the gene function through the determination of mutant characters to obtain a phenotype mutant, which determined by genotype was very important in biology research. 3 methods to construct gene mutant, such as transposon inserting mutant, homologous recombination and site-directed mutagenesis.The method of transposon insertion was used to construct the mutant library, homologous recombination was used to study the gene function, and site-directed mutagenesis was used to study the protein function. 3 methods emphasize a different aspect respectively. The combination of homologous recombination and site-directed mutagenesis would to be a new direction for gene mutations. Finally, the defects and shortcomings of gene mutation technology were discussed in this paper. The technology was proposed to be improved in the future.