Abstract:

In order to solve the problem of tissue culture seedlings leaves faded red return green in the rapid propagation system of Begonia fimbritipula Hance on Dinghushan Mountain. Adventitious bud with corm was used as explants, using the sucrose concentration screening method, researched of anthocyanin synthesis of Begonia fimbritipula Hance. Results showed that: In the basic culture medium (MS＋NAA 0.3 mg/L＋IBA 0.2 mg/L＋carrageenan 7.0 g/L), appropriate raised sucrose concentration at 45-60 g/L，was in favor of seedling growth more stronger and anthocyanin accumulation, relative content of anthocyanin was 0.417μmol/(g?FW). But when concentration of sucrose was ≥75 g/L, the growth of the seedlings was obvious inhibition.