Abstract: In order to clarify the change process of groundwater level in process of single wells withdrawing water in the hinterland of Taklimakan desert, and providing theoretical basis for sustainable water supply, the change of water level was observed in the pumping process at Tarim Desert Highway Shelter-forest Ecological Project No.69 well. Using these experimental data, several hydrogeological parameters of aquifer were determined by empirical Formula. The Law of Temporal and Spatial Variation in the process of pumping water can be demonstrated by experimental data analysis. The result shows that: The calculated permeability coefficient was 13.32 m, influenced radius was 332.04 m, and specific yield was 5.08% . In the two stress periods of pumping water, groundwater level of rapid decline and rapid rise can be completed at the beginning of its 11 minutes during Pumping. Groundwater level changes to the slow phase after 11 minutes. The water level could be restored to the level before pumping, and, the continuous decline of the natural underground water level was not found.