Abstract: The aim was to provide the theoretical basis for improving the yield and utilizing the adequate fertilizer to cultivate Cyathula officinalis. Three accessions of C. officinalis, A3, B1 and B2 from Sichuan were sampled regularly random at different growing time, the contents and the distribution and accumulation characteristic of N, P and K of two parts (aerial and underground) were tested. The dry matter weights were also determined. The dry matter accumulation of all parts from C. officinalis increased steadily with the growing periods. The contents and the distribution and accumulation characteristic of N, P and K of two parts were diversity in different growing periods of C. officinalis. In the whole growth period of C. officinalis, the accumulation of N, P and K was N>K>P, and the content of these nutrient elements were the highest at vegetative growth phase, starting decreasing from reproductive stage. For C. officinalis, the accumulation of N was at vegetative growth phase, P and K were at reproductive stage. For production of 100 kg dry matter, 4.90-6.69 kg N, 0.71-0.79 kg P2O5 and 2.98-5.16 kg K2O were needed. To assure the meet of growth rapidly at August and September, C. officinalis from Sichuan should be given enough base fertilizer in field cultivation. As the important periods at reproduction stage, the speeds of dry matter accumulation were the fastest. So, phosphorus and potassium should be supplied during the late of August (before reproduction stage).