Abstract: Post-anthesis nutrient regulation has been regarded as a promising way to delay premature senescence and increase kernel size in agronomic practices. In order to determine the effect of post-anthesis different nutrient regulation strategies on both dry matter production and N translocation for high yielding winter wheat, in addition to providing the theoretical support on post-anthesis nutrient regulation for high yielding winter wheat, we conducted a research in field involving post-anthesis once N fertilizer, fourth N fertilizer, spraying KH₂PO₄ to investigate effect of the each treatment on grain-filling and remobilization of N. Under favorable high yield conditions, control (without inputs of N) attained 8.7 t/hm2. Even though under high yield conditions, post-anthesis nutrient regulation could increase yield (once N fertilizer 8.9% , fourth N fertilizer 9.9% , spraying KH₂PO₄ 11.6% ) in comparison with control. Compared with control, post-anthesis nutrient regulation not only increased post-anthesis dry matter and N uptake, but also promoted remolibition of assimilate and N stored in vegetative tissues. Consequently, it was proposed that, assuring the adequate N situation in combination with spraying KH₂PO₄ could mitigate earliness induced by stress and improve the grain filling process.