Biological Effects of Sunflower Seeds Implanted by Carbon Ion

doi:10.11924/j.issn.1000-6850.2012-2075

Abstract

Abstract: The aim was to study the growth and genetic variation effects of carbon ion implantation and implantation times on different kind of sunflowers. Carbon ions were implanted into‘Ba-sunflower138’,‘Ba-sunflower136’and‘Ba-sunflower118’seeds in dose of 5×1013 C/cm² respectively, planting and harvesting the seeds to get irradiated again. The author continued the field test, observed the growth conditions of different Ba-sunflowers, and analyzed the genetic variation with RAPD. The germination rate and a number of traits like plant height, stem diameter, leaf number and yields of‘Ba-sunflower138’had a significant improve, while the twice-irradiated group just the opposite; the variation of‘Ba-sunflower136’and‘Ba-sunflower118’was not significant. At the molecular level, the genetic distance with the control group of once and twice-irradiated groups was 0.1111, 0.1087 in‘Ba-sunflower 138’; 0.0680, 0.0303 in‘Ba-sunflower136’and 0.0625, 0.0435 in‘Ba-sunflower118’. Carbon ion implantation had a significant effect on the growth and development of‘Ba-sunflower138’, and its effect varies with irradiation times, at the same time, it could cause genomic variation in the three kinds of sunflowers.

References

[1] 张明.国内外向日葵育种概况及动向[J].黑龙江农业科学, 2010(6):149-151.
[2] 金梦阳,危文亮,严新初.我国向日葵育种研究现状及发展对策[J].内蒙古农业大学学报,2008,29(3):232-236.
[3] 王鹏冬,杨新元,白冬梅,等.油葵杂交种含油率与地理位置的关系研究[J].中国油料作物学报,2002,24(4):38-42.
[4] 牛庆杰,李伟,李慧英,等.向日葵耐盐碱材料筛选的新途径[J].吉林农业科学,1998(3):27-28.
[5] 刘海学,李雪松,苏明,等.向日葵耐盐突变体筛选[J].内蒙古民族大学学报,2008,23(2):161-163.
[6] 王庆钰,孙云德.油用向日葵(Helianthus anuus L.)皮壳率的遗传研究[J].中国农业科学,1993,26(5):193-197.
[7] 张义,孔凡甲.向日葵子实含油率的遗传[J].吉林农业科学,1989(4): 60-63.
[8] 张义,孔凡甲.向日葵子实体积重量性状遗传及选择效果[J].中国油料,1989(4):36-40.
[9] 崔良基,王德兴.油用型向日葵杂交种主要性状及与产量关系研究[J].杂粮作物,2003,23(2):89-92.
[10] 贾瀚超,张斓,周云龙.低能离子注人技术在生物学中的研究和应用[J].生物学通报,2007,42(2):2-5.
[11] 朱蕊蕊,杨姗姗,王宇钢等.H离子注入耧斗菜干种子对萌发率的影响[J].北方园艺,2009,10:88-90.
[12] 梁前进,胡玉连,张根发.拟南芥(Arabidopsis tha liana)氮、碳离子注入诱变效应分析[J].生物物理学报,2002,18(2):251-255.
[13] 刘凰,邹东旺,唐记平,等.离子注入技术及其在籽莲育种上的应用研究[J].长江农业,2009,16,54-56.
[14] 陆挺,谢立青,朱凤绥,等.离子注入植物种子的生物效应研究[J].核技术,1994,17(7):443-448.
[15] 张晓旭,胡莹,刘静,等.碳离子注入向日葵种子生物学效应的研究[J].中国农业科技导报,2010,12(5):123-129.
[16] Yang X C, Qu Y, Li W J. Study of Salt Tolerant in Oil Sunflower Induced by Heavy Ion Irradiation[J]. IMP & HIRFL Annual Report, 2010(0):139.
[17] Liu Q F, Li W J, Qu Y, et al. Effects on Seedling Growth and Antioxidant Enzymes Activity of Oil-sunflower Irradiated by Carbon Ion Beams[J]. IMP & HIRFL Annual Report,2010(0):138.
[18] 董贵俊,张卫东,陈双燕,等.N_注入引起蛋白质组变异研究初报[J].生物物理学报,2004,20(4):269-273.
[19] Williams J G K, Kubelik A R, Livak K J, et al. DNA Polymorphisms amplified by arbitrary primers are useful as genetic makers[J]. Nucleic Acids Research,1990,18(22):6531-6535.
[20] 王志敏,牛义,宋明,等.RAPD技术在蔬菜研究中的应用[J].西南园艺.2005,33(5):21-22,29.
[21] 王广甫,董平.GIC41172×1.7MV串列加速器高能注入靶室的改进[J].北京师范大学学报:自然科学版,2002,38(5):628-631.
[22] 王卫东,刘磊安,王燕,等.氮离子注入的拟南芥 M1 代 RAPD 分析[J].西北农林科技大学学报:自然科学版,2004,32(5):45-48.
[23] 陈若雷,宋道军,余增亮,等.RAPD分析 N_注入紫花苜蓿种子后幼苗基因组 DNA变异[J].高技术通讯,2001,11(11):12-16.
[24] 魏胜林,刘竞男,王陶,等.N+注入对甘草种子萌发和根发育效应及作用机制[J].草业学报,2004,13(5):112-115.
[25] 汪宁,许峥,张丰收,等.离子注入线辣椒 F2代表型观察及变异分析[J].中国农学通报,2011,27(2):109-113.
[26] 何志美,郭军战,包振华,等.离子注入诱变鸡冠花 M1代形态变异的研究[J].北方园艺,2010(10):155-158.
[27] 肖尊安,武菊英,孙振元.低能碳离子注入野牛草种子的变异分析[J].北京师范大学学报:自然科学版,2004,40(4):518-522.