Abstract: The purpose of this paper is to find an identification method of the developmental Pulsatilla cernua and discuss the relevance between the developmental period of Pulsatilla cernua microspore and the morphology of flower organ. So the paraffin section solutions were selected for use in investigating the developmental Pulsatilla cernua and the relevance between the microspore development and the bud size had been studied. The results showed that in Pulsatilla cernua, each anther had four pollen sacs, the microspore mother cells belonged to the simultaneous type of meiosis, and microspore tetrads were tetrahedral. The mature pollens belonged to the two-cell type with three germination aperture. The pollen sac wall was composed of four 1ayers, epidermis, endothelium, middle layer, and tapetum. The tapetum belonged to glanduar tapetum type with multinucleate phenomenon. Microspore development also has correlativity with the morphological characteristics of buds and the color of anthers. The buds 1.10-1.20 cm in length, the filaments 0.4-0.5 cm in length, with purple calyx and yellow anther were discovered to contain microspores at the late-uninucleate. Microspore development of the same bud in Pulsatilla cernua showed continuity.