Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (3): 173-179.doi: 10.11924/j.issn.1000-6850.2012-2278

Special Issue: 生物技术

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Construction of Plant Expression Vector of AtFT Gene

  

  • Received:2012-06-25 Revised:2012-07-22 Online:2013-01-25 Published:2013-01-25

Abstract: FT was an important gene involved in controlling plant flowering time from Arabidopsis thaliana. This research used FT gene expression characteristics, through cloning FT gene from Arabidopsis thaliana, plant expression vector pCAMBIA2301-FT and pCAMBIA2300-FT:GUS which had a CaMV 35S promoter and GUS reporter gene, were successfully constructed. The somatic embryos in Hevea brasiliensia were transferred by agrobacterium-mediated transformation, and the instantaneous expression of somatic embryos was observed using GUS histochemical staining. The result showed the two plants expression vectors were effective. It laid the foundation for further studying the function and application of FT genes in Hevea brasiliensia.