Abstract: To discover the antifungal activity of the extracts from Phellinus linteus. We used the growth rate method measuring antifungal action to seven kinds of plant pathogen by Phellinus linteus different fermentation time fermentation product, the fermentation product was treated by different way, get the polysaccharide from the ethanol concentration, compare to antifungal activity. The results showed that antibacterial substances from Phellinus linteus have growth inhibitory effects on seven pathogenic bacteria; fermentation time was 20 d, the antibiotic compound had the highest inhibitory rate to Cytospora chrysosperma. The antibiotic compound from Phellinus linteus almost exists in supernatant of acoparia-alcohol extract; 30% alcohol-precipitation concentration (concentration was 50 mg/mL) was highest in all. Phellinus linteus contained the antifungal activity component and had the potential became the new biological pesticide.