Abstract: Wheat Pollen polysaccharides on inhibition of microbial. Wheat pollen was carried out with cell-wall-broken treatment through temperature difference wall-broking method and enzymolysis wall-broking method in this study. Active polysaccharide was extracted from cell-wall-broken pollen and was purified by use of methyl alcohol fractionated precipitation method. Experiments using two-factor randomized block design, and the inhibitory effect of the polysaccharides of wheat pollen analysis of variance and multiple comparison tests. The results indicated that Wheat pollen polysaccharide had very significant inhibitory effects to bacteria and had significant inhibitory effects to fungi. The polysaccharide purified by 35% of methyl alcohol with 3000r/min centrifugation had the best inhibitory effect on bacteria and fungi. Methanol concentration of 30%, 4000r/min centrifuge and the inhibitory effect of active polysaccharides from the bacteria to 30% methanol concentration, 3500r/min centrifugal purification process significantly; other methanol concentration, obtained by the centrifugal rotation of the purification process activity polysaccharides on the bacterial inhibition effect.