Abstract: The study aims to explore the drought resistance index and physiological and biochemical indexes at millet seed germination, which provide more theory basis for early appraisal of drought resistance. The study on drought resistance of twenty maize varieties at germination stage was conducted through adopting PEG solution simulated soil drought treatment. Using GDRI as basis to screen millet with drought resistance at germination period, twenty maize varieties were classified into five categories. The results showed that: germinating energy and germination percentage were decreased, the growth of young roots and buds were depressed at various levels under osmotic stress. There were positive correlations between GDRI and germinating energy, germination percentage and bud length (r=0.90**; r=0.73**; r=0.63**), roots length and lateral root number also had a positive correlation with GDRI to a certain extent (r=0.48*; r=0.51*). While coleoptile length did not correlate significantly with germination drought resistance index. Analysis showed that, germinating energy could be used for the first indicator of millet germination stage; germination percentage and bud length could be used for the second indicator; roots length and lateral root number could be used for the third indicator.When evaluating the GDRI of millet and other five indicators, drought resistance index can be used as a main marker and the other five indexes as supplements.