Abstract: In order to reveal the structure and functional characteristics of EeDREB2, and provide important candidate genes for transgenic wheat, the cDNA of EeDREB2 was cloned in Elytrigia elongate. The results showed that the cDNA of EeDREB2 was 1035 bp and encoded 344 amino acids, encoding a hydrophilic amino acid with 20 phosphorylation site. The iso-electric point and the molecular weight was 4.85 and 37485.49 Da. Subcellular localization predicted EeDREB2 gene located in the nucleus. Gene expression profile analysis found that EeDREB2 gene expressed in roots, stem, leaf, flowers, seeds, and was more highly expressed in leaf. The homology analysis showed that EeDREB2 shared high homology to TaDREB4B. The cloning of the EeDREB2 gene provided effective resources to resistance research of transgenic wheat.