Abstract: To develop liquichip method for detecting Potato virus A (PVA), the nucleotide sequence of PVA was analyzed by using the software Primer Premier 5.0, and specific probe labeled with biotin was prepared and coupled with fluorescence-coded microspheres, which was used for hybridization reaction to RT-PCR products of PVA. Liquichip detection method for PVA was established by using Bio-Rad Liquichip to detect fluorescence signal in the reaction system. The specificity of this method was evaluated by applying the proposed method to detect four viruses infecting potato, TBRV, Potato spindle tuber viroid (PSTVd), Potato virus A (PVA) and Tomato spotted wilt virus (TSWV). The results demonstrated better specificity to PVA and no reaction with other three viruses. The sensitivity of the method was 1 pg/μL of total RNA, which was equal to the conventional RT-PCR gel electrophoresis method.The successful detection of PVA in potato samples suggested the feasibility of this procedure for routine testing