Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach

doi:10.11924/j.issn.1000-6850.2012-3016

Abstract

Abstract: In order to investigate the control mechanism of peach flowering, the ap2 gene of peach was cloned. The peach (Prunus persica) cultivar‘Dongfeng’was used as material. In silico cloning PCR and RACE were employed to cloning complete cDNA sequence. The function was analyzed by bioinformatics methods. The gene had 1767 bp nucleotides and an ORF frame with 467 amino acid residue. The deduced protein had a molecular weight 52.0 kD, an equipotential point 8.73 and two copies of AP2 regions. PpAP2 was located in scaffold_6 in peach genome, and a SSR marker, namely AP2M anchored in it. Amino acid sequences alignment revealed that high similarity presented between PpAP2 and AP2 from other plant species, in which the highest homology found between PpAP2 and Malus×domestica AHAP2. The coincidence between AP2 genes revolution and species revolution was concluded.

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