Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (7): 111-116.doi: 10.11924/j.issn.1000-6850.2012-3086

Special Issue: 生物技术

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Cloning and Sequence Analysis of Auxin Response Factor of Seedless Litchi

  

  • Received:2012-09-10 Revised:2012-11-20 Online:2013-03-05 Published:2013-03-05

Abstract: To obtain the auxin response factor gene (ARF) that was differentially expressed in ovule degeneration of seedless litchi, in this study the gene was cloned and analyzed. The EST encoding ARF was obtained from young abortive ovule of seedless litchi via SSH (suppression subtractive hybridization), extracted high quality RNA and cloned the total cDNA sequence by SMAT-RACE method, bioinformatics analysis showed that it included an 2550 bp open reading frame which encoded a predicated protein with 849 amino acids, moreover, it had B3, Auxin_resp and AUX_IAA conserved region and functional domains, indicated that it was a new member of the ARFs gene family and got the closest relatives with Mangifera indica.