Abstract: In this study, the rachis was used as explant to induce bulblets in vitro of Chongming daffodil (N. tazetta var. chinensis), and the bulblets were inoculated in the medium added different concentration and proportion of hormone, sugar, and activated carbon (AC). Then the bulblets’reproducibility and diameter were analyzed to find the influent factors for obtaining the suitable conditions of multiplication. The results were showing as follows: (1) the medium added with 10 mg/L BA, 5 mg/L NAA was fit for bulblets induction from rachis, and got highest induction rate as 171.3%. (2) The medium added with 5 mg/L BA, 1 mg/L NAA, 40 g/L sucrose was optimum for propagation, and the induction rate reached to 175.0%, and the diameter was 0.41 cm. (3)Within the specific limits, 40 g/L sucrose was helpful for bulblets proliferation and growth. (4) AC had no affection on bulblets’diameter, and reduced the propagation rate remarkably, should not be added in medium.