Abstract: For different areas potato high quality and high yield cultivation to choose appropriate variety, metallograph method and SPAD-502 plus were used to measure stomata density and relative content of chlorophyll in three varieties of potato. The research results showed that, potato stomata density gradually changed in growth period, and decreased in full-bloom stage; potato relative chlorophyll content (SPAD value) change trend were the same to potato stomatal density basically. Through the experimental study concluded that, drought resistance higher variety was ‘Qingshu 168’, nitrogen needed higher variety was ‘Qingshu 2’.