Abstract: Male sterility in plants is a widespread phenomenon，male sterile crop is the main way of heterosis. It has already widely studied in genetic breeding, physiological , cytologyand biochemical and molecular biology. In recent years due to the corresponding proteomics research technology continues to evolve, the technology in the study of male sterility in plants widely used，and this technology provides an important basis for analysis of the molecular mechanism of plant male sterility. This paper reviews recent proteomics research on cytoplasmic male sterility and genic male sterility, we believe that male sterility in plants has contact with the raised of the expression of some proteins, decline, missing, or some new proteins’ expression, these proteins are involved in the metabolism, transcription, signal transduction, protein synthesis, starch synthesis, apoptosis and other important physiological processes, and it is pointed out that the proteomics deficiencies in the plant male sterility and the development direction.