Abstract: In order to change the high status of fertilizer application amount in the grain production, the experiment with single factor design to study the feasibility of reducing fertilizer application by using the bio-fertilizer on wheat in Hexi oasis. The results showed that, using the bio-fertilizer could improve the utilization rate of fertilizer, water and light energy. Because of the biological fertilizer combined application, when fertilizer application amount was reduced to 15% and 30% on the current high fertilization level (N: 180 kg/hm2, P2O5: 90 kg/hm2), wheat yield was 6428 kg/hm2 and 5943 kg/hm2, nitrogen utilization rate was 30.75% and 31.66%, phosphorus utilization rate was 22.65% and 22.74%, water use efficiency was 1.26 kg/m3 and 1.18 kg/m3, light energy utilization rate of growth period was 0.69% and 0.68%. Trials had been obtained which was feasible that the usage of fertilizer reduced the magnitude of less than 15% in actual production under the condition of with the application of high-quality biological fertilizer.