Abstract: In order to fully utilize the pupa resources, this article had studied the dual-pupa protein hydrolysis of the optimal conditions，determined the impact of the main factors enzyme effect. A fresh study by blanching chrysalis, to fat, enzyme, the deployment of processes such as protein drinks made from the process, eliminate the chrysalis of the Browning and condensate Shen phenomenon. By three factors and three levels orthogonal L9(33) Test methods, respectively, the pupa protein enzyme temperature, hydrolysis time, the amount of experiments and analysis, the double-pupa protein hydrolysis the optimum conditions. The results showed that: the best conditions for the enzyme trypsin: pH value of 7.0, the temperature of 45℃, hydrolysis time 5h, the amount of 1 g. Papain: pH value of 6.0, the temperature of 70℃, hydrolysis time 4 h, the amount of 1 g. Conclusion trypsin into the pupa protein enzyme papain process, will enable enzyme solution of ammonia nitrogen content have significantly improved. The use of its enzyme preparation can be diluted into amino acid drinks with a unique flavor, texture soft, delicate features and nutrient-rich, such as certain medicinal value, is a function of the new health drink. Its total number of bacteria, coliform bacteria, pathogens in conformity with the relevant health indicators. Amino acid drinks in the total sugar (sucrose) content of 14%, acidity (citric acid) content of 0.68 g/L, clear transparency of their products, Tiansuan palatability, with the physical and chemical testing beverage health indicators.