Abstract: Abstract Objective: Rhizosphere soil extract was analyzed to get some primary information about Salvia miltiorrhiza root exudates. Method Ethyl acetate and Hexane was used to extract rhizosphere soil of S. miltiorrhiza, the extract was filtrated and concentrated, then analyzed by gas chromatography-mass spectrometry(GC-MS). Results: About 27,25 peaks were observed and identified from Ethyl acetate and Hexane extract respectively, which consist of hydrocarbon, alcohol, aldehyde, ester, organic acid, and so on. The peak areas were normalized to calculate the relative contents of each component. And among all these components, hydrocarbon and alcohol have the highest proportion in Ethyl acetate extracts; phenol, hydrocarbon and alcohol have the highest proportion in Hexane. This experiment result proves that allelopathic substances exist in root system. Conclusion Many organic compounds are contained in S. miltiorrhiza roots rhizospere soil, according to GC-MS analyzing result of soil extract, which are possible that that these substances are excreted by S. miltiorrhiza roots. Further research about the action mechanism of these substances is remained. Keywords: Salvia miltiorrhiza Bge; rhizosphere-soil; root exudates; GC-MS