Abstract: In order to research the difference of Cd, Zn and As accumulation in different hybrid rice cultivars, 30 different hybrid rice cultivars were cultivated in a heavy metal and arsenic polluted paddy field which located in a mine zone of southern Hunan Province. The results showed that: there were certain differences of accumulation Cd, Zn and inorganic As between different genotypes of hybrid rice cultivars. The brown rice contents of Cd, Zn and As in 30 different hybrid rice cultivars were 0.28-0.83, 24.13-34.16, 0.45-0.77 mg/kg, respectively. Cd accumulation in brown rice of different genotypes of hybrid rice cultivars has significant difference, while Zn and As accumulation in brown rice is small differences. The significant difference of Cd content in brown rice exist between the two-lined hybrid rice and three-lined hybrid rice (P<0.05), and the differences of Zn and As contents in brown rice was not significant. Therefore, depending on differences of heavy metals accumulation in different hybrid rice cultivars, it indicated that the selection the pollution-safe cultivars were possible.