Abstract: Abstract: A desulfurization strain HDBS-1 was selected which can degrade dibenzothiophene (DBT)，which was identified as Enterobacter sakazakii. This strain can convert dibenzothiophene to 2-hydroxybiphenyl (2-HBP) through specific desulfurization pathway. The best desulfurization conditions have been researched for getting a higher ability Desulfurization strain .w e find the best optimum conditions are that the cultures containing 25mL in every 250mL medium, 6% inoculated, 30℃ in temperature.In this optimum medium, the obtained strain HDBS-4 raised its ability to convert dibenzothiophene to 2-hydroxybiphenyl (2-HBP) , which gave a fold increase compared with usual condition.Even 12 times higher than the ability of the original strain.