Abstract: In order to study the response of soil enzymes of wheat season to soil fertility levels, a long-term experiment with four fertilization patterns (CK, NPK, NPK+medium manure, NPK+high manure) started in 2002 at Changshu Agro-ecological Experiment Station of Chinese Academy of Sciences (31°33′N, 120°42′E) to investigate the effects of different fertilizations on soil enzyme activities at panicle and harvest stage of wheat. Four enzymes closely related to soil nutrient and energy cycling, i.e. urease, invertase, acid phosphatase and polyphenoloxidase, were selected to analyze. Results showed that: the treatments of fertilization had significantly higher soil enzyme activities than treatment CK except the polyphenoloxidase, and the treatment NPK+medium manure had the highest enzyme activities among four treatments. Correlative relationship between soil enzyme activities and crop yields was better than that soil nutrients and crop yields. The trends of soil enzyme activities of different fertilizations were similar in the two wheat-growing stages.