Abstract: H2S was considered as the third gas signaling molecule and invoved in many plant physiological processes. This article summarized the sources of hydrogen sulfide, advancement of H2S in regulating seed germination, root and leaf development, stoma closure, photosynthesis, substance metabolism, senescence of flowers, and the effect of relieving abiotic stress, such as salt, high temperature, osmosis, drought, heavy metals and so on. Meanwhile, this article also provided a possible mechanism of H2S regulating plant growth and development, and the future development in this field was prospected.