Abstract: Abstract：The N gene, cloned from wild tobacco（Nicotiana glutinosa）, is a type member of the Toll-IL-1 receptor homology region (TIR)-nucleotide binding site (NBS)-leucinerich repeat region (LRR) class of R genes and confers resistance to tobacco mosaic virus (TMV). The N gene was isolated by insertional mutagenesis using the Activator (Ac) transposon of maize，and N-TMV interaction is one of the earliest and most studied models of plant-pathogen interaction. In this article, the research progress of N gene in structure, expression, action mechanism were reviewed from the transcription products, expression characteristics, temperature sensitivity, corresponding avirulence gene and other aspects; The efficient using and achievements of N gene in TMV resistance breeding were summarized; And the prospect of efficient using of N gene was gave from three aspects, which is the mechanism research of resistant to TMV, identification and utilization of germplasm resources.