Abstract: The induction and differentiation effects of various concentrations of ployamines, cerium nitrate and lanthanum nitrate on Erica carnea’s subculture test-tube seedlings were studied to provide theoretical basis and experimental basis of ployamines and rare-earth elements application in plant tissue culture. The method applied to plant tissue culture, regarded Erica carnea’s subculture test-tube seedlings as an explant, were discussed the effect of variable concentration of ployamines, Ce(NO3)3 and La(NO3)3 on the introduction of the subculture test-tube seedlings. The results showed that the average differentiation times, the average height of differentiation plantlet and embryogenic callus induction frequency of Erica carnea on basic medium (1/2 WPM+ZT 1.0 mg/L+IBA 0.3 mg/L) supplemented with 1.0 mg/L spermdine were 3.89, 3.11 cm and 98.30% respectively, which were significantly better than other treatment demonstrated by analysis of variance and multiple comparisons results; basic medium with 1.0 mg/L cerium nitrate showed the average differentiation times 6.9, the average height of differentiation plantlet 4.0 cm, and embryogenic callus induction frequency 98.70%, which were significantly better than other treatment demonstrated by analysis of variance and multiple comparisons results. Meanwhile basic medium with 0.5 mg/L lanthanum nitrate gave the highest embryogenic callus induction frequency 100%, the average differentiation times 5.5, the average height of differentiation plantlet 3.89 cm, which were also significantly better than other treatment indicated by analysis of variance and multiple comparisons results. The low concentration of ployamines and rare-earth elements acted as a stimulus to the differentiation as well as growth of Erica carnea’s subculture test-tube seedlings, otherwise, the high concentration caused the inhibition of the differentiation and growth of Erica carnea’s subculture test-tube seedlings.