Abstract: In order to analysis and compare the effect of pregnancy and amputation of tail on temperature choice in the female of Takydromus amurensis, this study was conducted in the June and August of 2012. Two populations of Takydromus amurensis respectively from Shangzhi (Heilongjiang Province) and Kuandian (Liaoning Province) were studied with the method of field capturing and laboratory feeding. Our results showed that Heilongjiang population of T. amurensis could make their own body temperature increase through the heat source thermostat, when the temperature of the external environment was lower (20℃). The mean temperature of the female was (32.49±0.705)℃ in the non-gestation period. However, in the pregnancy period, the mean temperature of the female was (33.90±0.674)℃, so the body temperature was significantly higher after the pregnancy (F1,61=65.600，P＜0.001). No matter the pregnancy period or the non-gestation period, the mean temperature of the female T. amurensis from Shangzhi (33.39±0.909)℃ was significantly higher than the female from Kuandian (32.91±1.021)℃. It showed that the ability of thermoregulatory was stronger in the area of the higher latitude. The mean temperature of the female from Kuandian was (31.94±0.590)℃, and it was (31.60±1.268)℃ from Shangzhi after amputation of tail. The difference was no significant between the two populations (F1,18=0.591, P=0.452), but the mean temperature was obviously lower than the individual have the complete tail (F1,18=9.854, P<0.05). The results showed that tail have the influence on thermoregulatory.