Abstract: [Aim] To identify whether transformed successfully Bar gene into T1 generation of transformed-plant flax. [Method] The Bar was transformed into flax using pollen tube pathway. The minimum lethal dose of glufosinate to T1 generation was screening by leaves smear the drug. Then, glufosinate-tolerant plants were also screened through drug spray in field, and Bar-positive detect of anti-drug plants were conducted by PCR. [Results] The minimum lethal dose of glufosinate to T1 generation was 100 mg/L. There were 44 T1 glufosinate-tolerant plants survived in field, and 8 Bar-positive plants were confirmed by PCR, which average transformation rate was 18.2%. [Conclusion] It is successful to transform Bar gene into flax using the method of pollen tube pathway. Key words: pollen tube pathway; glufosinate; bar gene