Abstract: In order to explore the ecological adaptability of the variety tobacco NC297 in different altitude, the continuous many years of the demonstration experiments with multiple spots were carried. The results showed that the NC297 has an extensive ecological adaptability in different altitude, and it has only better tobacco coordination with its leaves output from 2016 to 2562 kg/hm2, resistance, quality and other agronomic traits similar to the performance of their original habitat, but a better resistance to fertilizers, strong disease resistance, high quality and appropriate output, easy to baking, high synthetic score of the appearance quality, a ratio of sugar and nicotine and a better radio of total sugar and reducing sugar also. It has more better aroma of sweet rhyme and richness than that of the check varieties K326 and Yunyan 87. It is a better suck quality with its qing scent style deserving to popularization in the medium or above fertility soil from altitude 1600 to 1800m. The most suitable planting density is from 15000 to 16500 plants/hm2, the suitable fertilizer ratio of N, P2O5 and K2O is one to one to three, total applied nitrogen of paddy land is from 105 to 135 kg/hm2, and that of dry land form 75 to 105 kg/hm2.