Abstract: The influence of different explants and phytohormone on the protocorm-like-body induction from Pinellia ternate was studied to establish a protocol of a rapid propagation of Pinellia ternate. ‘Yuanzhu Pinellia ternate’of Dafang in Guizhou Province were used as explants to conduct protocrom-like bodies induction, differentiation and domestication transplanting technology test research. The proper protocrom-like bodies’ induction medium was MS+6-BA1.0 mg/L+NAA0.3 mg/L. Induction rate and the multiplication factor were 86.67% and 17.40. Leaves and petioles formed protocorm-like-body (above 50%) on the medium of the MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, and the biggest proliferation multiple was 12.80 and 8.6, respectively. 0.5 cm, 9.4 root number/ plant, 3.71 cm root length tissue culture seedlings which has high survival rate and grows well was the best tissue culture seedling for transplanting. The best growth in the matrix was based on the ratio of soil:perlite=1:1(V:V). Protocorm-like-body of Pinellia ternate could be induced in the suitable ratio of plant hormones from the right explant and high frequency regeneration system of Pinellia ternate could be established, which laid a foundation for Pinellia ternate germplasm resources conservation, gene engineering breeding, medicinal secondary metabolite production and so on.