Abstract: New ramie variety 0501 was used in this research, in order to exploring the comprehensive effects and nutrition physiology of fertilizer and plant density on ramie yield and quality. Orthogonal rotated combination design was used to study the nutrient accumulation and distribution in ramie. The main results showed that: nitrogen significantly effected on NPK contents in ramie plant, nitrogen fertilizer promoted nitrogen accumulation, but decreased the accumulation of phosphorus and potash in ramie plant. Potash fertilizer can increase the amount of phosphorus and potassium in the ramie leather. There are some differences in nutrition distribution between first and second season of ramie plant.