Abstract: In order to accelerate early growth and quick tillering of transplanted tobacco plants, experimental material ‘K326’ seedlings were floated in greenhouse. Spraying 0, 0.5, 1.0, 1.5 mmol/L salicylic acid on foliar respectively when the seedlings with three or four leaves. Then the grown seedlings were transplanted into field. The results showed that transplanted tobacco plants can adapt to the changed environment quickly after treated by salicylic acid because it can increase the activity of antioxidant system and then remove reactive oxygen species ROS timely and effectively. So salicylic acid can promote the development of root system rapidly, strengthening the root vigor and increasing root-shoot ratio. The result is rosette stage was shorted; early growth and quick tillering were accelerated of transplanted tobacco plants. All of those were beneficial to tobacco plants subsequently grow and accumulate dry matter rapidly. Effects of four different salicylic acid concentrations were different. The effects of 1.0 and 1.5 mmol/L concentration were better than 0.5 mmol/L.