Abstract: In order to solve the problems of the virus-free seed potato of Guangxi Province, the breeder’s seeds of Feiwuruita were used of the experiment. In this experiment, the potato seedlings were treated and cultivated in water by different density and different times of NAA、IBA、GGR and NAA 16 mg/L+ IBA 50 mg/L+ GA3 35 mg/L, observed the morphologica of the seeding. Results: In the hormone of NAA, the rooting rate was 100% immersed with 16 mg/L NAA for 20s, which was higher than CK (83%). The rooting rate was 100% immersed with 50 mg/L IBA for 30 min, which all the characters were better performance and was higher than CK (86%). In the hormone of GGR, the rooting rate was 98% immersed with 200mg/L GGR for 30min, which was higher than CK(87%), and the rooting rate was 98% disposed by three hormones, were immersed in 16 mg/L NAA, 50 mg/L IBA and 35 mg/L GA3 for 30min, which was higher than CK(87%). Conclusion: The results show that the optimum concentrations of IBA to water cultivation were 50 mg/L and 30 min intruded into solution, which started rooting six days later with healthy roots, and the rooting rate was 100%.