Abstract: [Objective] This study is to research for the composition and distribution of endophytic fungi in Yunnan hemp 1. [Method][Result]531 endophytic fungi strains (138 from stem; 393 from leaf) were isolated from stem and leaf samples of industrial hemp (Yunnan hemp 1) collected from different sites, and identified by morphological and molecular methods. The isolated endophytic fungi were divided into 29 genera (22 genera from stem; 24 genera from leave),of which 18 genera were found both in stem and leaf. Colletotrichum was the dominant genus with dominance index 40.11%. The isolation rate of endophytic fungi in stem was evidently lower than that in leaf. Overall, the highest isolation rate was got in the samples from the basal part of hemp plants. The diversity index of endophytic fungi in hemp from the four sampling sites varied from 1.84 to 2.46. The similarity coefficients were high among all sampling sites, but they were low between stem and leaf from the same site. [Conclusion]The results showed that the host specificity and diversity of endophytic fungi in industrial hemp (Yunnan hemp 1) were low.