Abstract: Main chemical components and sensory quality of 42 eco-organic tobacco samples from 6 grades and 7 production sites in Guizhou Province (Guiding, Xingyi, Tianzhu, Weining, Wuchuan, Daozhen, Zunyi) were analyzed to study relationship between main chemical components and sensory quality. The results showed that chemical components of samples of each production site were more coordination. Two sugar ratio substantially was in the appropriate range. The difference of the nicotine content between different areas was little and the nicotine and chlorine content of the upper part of leaf in the multi-region was lower. The sensory quality and tobacco comprehensive quality were better. The strength was declined, especially Zunyi, Daozhen and Wuchuan and the fragrance of the tobacco in Weining was more significant. The aroma quality, aroma quantity, flammability, gray, and smoking total score of the measured samples were highly significant negative correlation with the ratio of sugar to nicotine and the ratio of nitrogen to nicotine. The biting taste was a significant positive correlation with total sugar and reducing sugar content.